Clinical Studies and Reports on the Antimicrobial Qualities of Colloidal Silver

The following PDF documents consist of a variety of clinical studies and independent research reports on the antimicrobial qualities of colloidal silver-related products including silver ions, silver compounds, silver nanoparticles, nanosilver compounds, silver-impregnated and silver-coated products, and many other forms of silver being used in a wide variety of ways, but almost all for the purpose of benefitting from the powerful antimicrobial qualities of silver itself.

These studies and reports are supplied for informational purposes only, and should not be construed in any way as providing medical advice or guidance. They cover a wide range of data on silver, including some surprising findings and discoveries regarding silver’s powerful antimicrobial, infection-fighting and disinfectant qualities.

These studies and reports are compiled here with the intent of giving the reader or researcher a general understanding of the many uses of antimicrobial silver, from a variety of viewpoints, both clinical and personal. The studies are not meant to convey efficacy of any particular colloidal silver product.

Because research into antimicrobial silver is ongoing, and because opinions and conclusions tend to vary depending upon the factors involved in different studies, these studies and reports should be considered merely as a starting point for your own research, and not be considered the final word on silver and its many antimicrobial, infection-fighting or disinfectant benefits.

Note to Publishers and Authors: If you feel any of these PDF files are on this site in violation of copyright, please inform me immediately by email, at, or at the phone number at the bottom of this page. These PDF files have been garnered from a variety of internet sources where they were found posted with no restrictions. They are here under fair use, and for non-commercial informational purposes only.

Colloidal Silver And Chitosan Stabilized Silver Nanoparticles On Mcf-7 And Hepg2

In this clinical study, published in the European Journal of Biomedical and Pharmaceutical Sciences, researchers tested the anti-cancer properties of colloidal silver and silver nanoparticles stabilized by chitosan. According to the study authors, “The present study was aimed to investigate the in vitro cytotoxicity effect of colloidal silver and chitosan stabilized silver nanoparticles against human breast cancer cells (MCF-7) and liver cancer cells (HepG2) towards the development of anticancer drugs.” Results: The silver treatments demonstrated “cytotoxic effect against MCF-7 and HepG2 cell lines.” The study authors concluded, “There was an immediate induction of cellular damage in terms of loss of cell membrane integrity, oxidative stress and apoptosis were found in the cell which treated with colloidal silver and chitosan stabilized silver nanoparticles.” In other words, both forms of silver caused liver and breast cancer cells to self-destruct.

Silver Nanoparticles Kill Lung Cancer Cells in vitro, Slow Tumor Grown in vivo

In this clinical study, titled “Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo,” published in May 2016 in the International Journal of Nanomedicine, cancer researchers from Rutgers State University of New Jersey and the Guandong University of Technology in China combined forces to test silver nanoparticles against lung cancer cells in vitro and in vivo. For the in vivo (i.e., live animal) testing, the researchers used immunodeficient mice into which lung cancer tumor was grafted. The researchers wrote: “Unlike other metal nanoparticles, AgNPs are nontoxic to human body at a lower dosage…the applications of AgNPs have increased in cancer diagnosis and treatment, not only as attractive targeted drug delivery vehicles or probes for early cancer screening, but also as a promising therapeutic molecule by itself. [In previous studies] AgNPs have shown potential cytotoxicity against various cancer cells such as lung cancer A549 cells, breast cancer MCF-7 cells, colon cancer HT29 cells, cervical cancer HeLa cells, and Dalton’s lymphoma ascites tumor…The present study was designed to explore the anticancer activities of our green-synthesized AgNPs in vitro and in vivo. These results further provide new approaches for treating lung cancer and other types of cancers by using silver-based nanoparticle therapies…AgNPs significantly suppressed the H1299 tumor growth in a xenograft severe combined immunodeficient (SCID) mouse model. The results demonstrate the anticancer activities of AgNPs, suggesting that they may act as potential beneficial molecules in lung cancer chemoprevention and chemotherapy, especially for early-stage intervention…Approximately, 50% of H1299 cells died when treated with AgNPs at the concentrations between 5 and 8 µg/mL.” In other words, not only did the silver nanoparticles suppress the tumor growth in the immunodeficient mice, causing tumor growth to slow dramatically as cancer cells died, but also a full 50% of the cancer cells tested in vitro (test tube) were killed at very low concentrations of silver (i.e., 8 ppm).

Anticancer Activity of Silver Nanoparticles Synthesized by the Seaweed Ulva lactuca Invitro

In this clinical study, published in the journal Open Access Scientific Reports in 2012, researchers tested silver nanoparticles against three different human cancer cell lines, i.e., laryngeal cancer cells (Hep2), breast cancer cells (MCF 7) and colon cancer cells (HT29). The researchers found the silver nanoparticles to be effective against all three cancer cell lines in test tube studies, without causing toxicity to normal cells. The researchers concluded, “The synthesized nanoparticles were potently cytotoxic against Hep 2 cell lines and mildly cytotoxic against MCF 7 and HT 29 cell lines… Cytotoxic effect is inversely proportional to the size of the bioactive compound AgNP.” In other words, the smaller the silver nanoparticles used, the greater the anti-cancer benefit documented.

Nanosilver Heals Liver Damaged by Powerful Toxin

Researchers from the Department of Environmental Biotechnology, Bharathidasan University, India, have demonstrated in an animal study that small amounts of silver can help heal liver damage caused by a particularly harsh liver toxin. The study is titled “Hepatocurative activity of biosynthesized silver nanoparticles fabricated using Andrographis paniculata,” and it was published in the journal Colloids and Surfaces B, Biointerfaces, in February 2013. The study reveals that when researchers used a very toxic solvent known as CC1(4) to damage the livers of mice, and then gave the mice silver nanoparticles, the silver cured the mice of the vast majority of the liver damage induced by the toxic solvent. The clinical researchers claimed in their study that at low doses, the silver “was effective in revival of all biological parameters to near normal in all intoxicated groups indicating the curing effects on CC1(4)-induced liver injury.” What’s more, the researchers attribute the amazing healing of the damaged mice livers to silver’s “strong antioxidant effect.” The researchers were so enthused by their discovery they even called nanosilver “hepato-curative,” meaning they feel it cured the liver damage induced by the toxic solvent.

Effect of Silver Nanoparticles on Common Bacteria in Hospital Surfaces

In this clinical study, published in June 2013 and titled “Effect of Silver Nanoparticles on Common Bacteria in Hospital Surfaces,” researchers from the Infectious and Tropical Diseases Research Center, University of Medical Sciences, Ahvaz, Iran, tested three different concentrations of silver nanoparticles – 100 ppm, 200 ppm and 300 ppm — on surface colonies of infectious microbes including MRSA, Pseudomonas aeuroginosa and Bacillus cereus. According to the study authors, each of the three dilutions of silver nanoparticles were able to produce “more than a 99 percent reduction” in bacterial surface contamination. While it took longer for the more dilute preparations to work, there were “no remarkable differences” between the three “when tested at 5,15, 30 and 60 minute disinfection intervals.” The researchers concluded: “Silver nanoparticles had appropriate effects in all three types of dilutions…After five minutes all disinfectants reduced the S. aureus colony count significantly (more than 99%), but none of them could bring the S. aureus colony count to zero. This rate was achieved following 30 minutes of disinfection with 300 ppm silver nanoparticles…For two other dilutions of silver nanoparticles, this rate was possible after 60 minutes of disinfection.” In short, whereas other clinical studies have demonstrated lower concentrations of silver nanoparticles to reduce bacterial count on hospital surfaces to zero after 24 to 48 hours, this study demonstrated that concentrations of 100 ppm, 200 ppm and 300 ppm silver nanoparticles could reduce the bacterial count by 99% after just five minutes of application, with a full 100% reduction in bacterial count being achieved within 30 minutes when using the 300 ppm nanosilver, and after one hour when using the 100 ppm and 200 ppm nanosilver.

Repeated Dose (28 Day) Administration of Silver Nanoparticles of Varied Size and Coating Does Not Significantly Alter the Indigenous Murine Gut Microbiome

In this clinical study, published in the journal Nanotoxicology in June 2016, researchers demonstrated that oral administration of silver nanoparticles capped with PVP or Citrate had negligible effect on gut bacteria in the murine (mouse) model — the implication being that silver nanoparticles delivered orally either through unintentional contact with consumer products, or through dietary supplementation, or as a substitute for antibiotics in animal feed would not harm beneficial digestive microbes. The researchers wrote, “We evaluated murine gut microbial communities using culture-independent sequencing of 16S rRNA gene fragments following 28 days of repeated oral dosing of well-characterized AgNPs of two different sizes (20 and 110?nm) and coatings (PVP and Citrate). Irrespective of size or coating, oral administration of AgNPs at 10?mg/kg body weight/day did not alter the membership, structure or diversity of the murine gut microbiome. Thus, in contrast to effects of broad-spectrum antibiotics, repeat dosing of AgNP, at doses equivalent to 2000 times the oral reference dose and 100-400 times the effective in vitro anti-microbial concentration, does not affect the indigenous murine gut microbiome.”

The Effectiveness Of Silver-Releasing Dressings In The Management Of Non-Healing Chronic Wounds: A Meta-Analysis

In this meta-analysis of eight randomized control trials, covering a total of 1,399 patients, and published in the Journal of Clinical Nursing in March 2009, it was shown that silver dressings significantly improved wound healing, reduced odor and pain-related symptoms, decreased wound exudates and had a prolonged dressing wear time when compared with alternative wound management approaches. Furthermore, the studies indicated an improvement in quality of life using silver dressings in wound management, with no associated severe adverse events. The meta-analysis confirms the effectiveness of silver dressings in wound healing and in improving patients’ quality of life.

Anti-diabetic Activity of Silver Nanoparticles Documented

In a clinical study titled “Green Synthesis of Silver Nanoparticles Using Tephrosia tinctoria and its Antidiabetic Activity,” published in the journal Material Letters, Volume 138, in January 2015, the researchers wrote: “The antidiabetic ability of the silver nanoparticles was tested and the results showed significant free radical scavenging ability, inhibition of carbohydrate digestive enzymes (a-Glucosidase and a-Amylase) and enhancement of Glucose uptake rate.” In other words, it appears the silver nanoparticles mopped up free radicals (which are often generated in excessive numbers in diabetic patients, and can damage the pancreas), inhibited the activity of enzymes that tend to increase blood sugar levels through the digestion of carbohydrates, and aided in glucose uptake — all critically important factors for diabetics.

Topical Colloidal Silver as an Anti-Biofilm Agent in a Staphylococcus aureus Chronic Rhinosinusitis Sheep Model

Here’s a very interesting abstract of an in vivo (animal model) clinical study published in April 2015 in the International Forum of Allergy & Rhinology, demonstrating the safety and effectiveness of colloidal silver in the treatment of Staphylococcus aureaus biofilms located in the sinuses of sheep. [A “biofilm” consists of a colony of bacteria living within a mucilaginous protective coating, making them more difficult for antibiotic drugs to reach and kill — ED]. Both a safety study, and an efficacy study were conducted. In the safety study, saline solution was used a control, while 30 ppm colloidal silver was tested against it as a flush for the frontal sinuses. The sheep sinuses were flushed with the solutions for 14 days (eight sheep total; four sheep tested with the control solution, and four with the colloidal silver solution). In the efficacy study, sheep with frontal sinus infections of Staphylococcus aureaus were tested for five days with either the control solution, or with colloidal silver solutions of concentrations ranging respectively from 30 ppm to 20 ppm, 10 ppm and 5 ppm. Again, there were four sheep in each group. The researchers state, “Blood silver level, full blood counts, and biochemical parameters were analyzed in both safety and efficacy studies…. Sheep treated with silver showed a significant decrease in biofilm biomass compared to saline control. Although average blood silver levels were higher in the treated groups compared to controls, blood counts and biochemical parameters were normal. Histology and ciliary structure analysis did not show any difference between control and treatment groups.” The researchers concluded, “Topical colloidal silver solution has effective antibiofilm activity in Staphylococcus aureus CRS in a sheep model and appears safe.”

Comparative Bacteriology Analysis: Particulate vs. Ionic Silver

In this December 2004 analytic lab study by Dr. John W. Roberts, PhD of the Natural-Immunogenics Corporation, the antimicrobial effects of two different forms of colloidal silver were tested. The ‘particulate’ (i.e., metallic) form of colloidal silver was tested against the (free) ‘ionic’ form of colloidal silver. The bactericidal effects of both forms of silver were compared by treating healthy cultures of two strains of Staphylococcus aureus separately. Each strain was treated separately with the ionic silver and with the particulate/metallic silver. The bacterial strains were also treated separately with equal amounts of HC1 (i.e., hydrochloric acid) added to the silver suspensions. Results: With or without the HC1 mixture added, the Staphylococcus cultures (both S-1 and S-2) on the plates were virtually eradicated in just four minutes when treated with the ionic silver. But the culture plates treated with the particulate/metallic silver showed only a slight reduction in the bacterial count in the same time-frame. The study authors concluded that the ionic form of colloidal silver is the active, antimicrobial form, while the particulate/metallic form is bactericidal only to the extent that there’s a small component of active silver ion content naturally occurring inside the particulate/metallic suspension. When the ionic silver component is removed, very little bactericidal effect occurs.

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